Core Facilities Genomics and Proteomics: Olink Proteomics

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Research

Protein analysis services using Olink technology

Our service portfolio includes high throughput protein analysis utilizing cutting-edge Olink® technology. Considered as a next-gen proteomics platform, Olink technology enables unparalleled throughput and specificity (Figure 1). Its consistent and reliable results have made it widely recognized and extensively cited in various research fields for protein profiling and biomarker development.

Key features of Olink proteomics

Exceptional specificity and sensitivity
Very low sample volume requirements
Low CVs
High throughput protein analysis

Protein measurement with Olink proximity extension assay (PEA) technology. (A) Samples are mixed with paired antibodies labeled with complementary DNA oligonucleotides. When the antibody pair correctly binds to its target protein in solution, the oligonucleotides hybridize to each other. (B) The hybridized oligonucleotides are amplified via PCR and analyzed with qPCR. (C) No DNA amplicons are generated if nonspecific binding occurs.

Supported Olink platforms

Olink platform	Library size (# proteins)	# Proteins measured at one time	# Multiplex panels	Sample consumption per panel	Quantification
Olink Target 96	~1100	92	15	1 µL	Relative
Olink Target 48 Cytokine	45	45	1	1 µL	Absolute
Olink Flex	~200	15 - 21	Mix-&-match	1 µL	Relative & absolute

last update:

23/01/2025

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