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Research

Possible analyzes

Routinely, we process the following samples:

Blood (EDTA, Heparin, Serum, Plasma)
Tumor tissue
Cerebrospinal Fluid

What can be done with which samples?

Sample material: Blood, EDTA
Possible Analyzes: DNA-Isolation, RNA-Isolation, TCR-Sequencing, HLA-Typing

Sample material: Blood, Heparin
Possible Analyzes: FACS, ELISpot, cell culture

Sample material: Serum
Possible Analyzes: Luminex, ELISA

Sample material: Plasma
Possible Analyzes: Luminex

Sample material: Tumor tissue
Possible Analyzes: culture of tumor infiltrating lymphocytes, FACS, DNA/RNA-Isolation, TCR-Sequencing

Required minimum quantity of samples:

This is to give an overview of how much sample material is needed in order to successfully carry out analyzes. This overview should help you to plan the sampling in your study. Of course, we are happy to advise you individually.

Calculation basis:
9ml blood (1 tube). Per 1ml blood about 1x10⁶ PBMCs can be isolated.
Per tube approx. 0.9 – 1x10⁷ PBMCs can be harvested.

ELISpot (4 groups per time point, triplicates):

ELISpot: PBMC
Cell amount: 1.5x10⁷Blood volume: 30ml (Heparin)

ELISpot: DC/TC
Cell amount: 4.5x107
Blood volume: 60ml (Heparin)

FACS (up to twelve colors per panel):

Per well: 3x10⁵ - 1x10⁶ PBMCs, depending on the goal; additional controls might be needed

Luminex (27 Cytokines/Chemokines per sample):

1 serum tube (approx. 7ml Heparin blood)

ELISA:

1 serum tube (approx. 7ml Heparin blood)

DNA-Isolation:

5ml EDTA blood

RNA-Isolation:

2 PAXgene® tubes (4ml blood)