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Cell Line Authentication
FAQ

Research

Human Cell Line Authentication FAQ

  • My cell line of interest is not included in the database, but I have recently acquired this cell line from the ATCC. Can my sample be tested along with the original reference material?
    • Yes, the sample can be tested by the human cell line authentication test, if your reference material is an aliquot of the original reference material obtained from the cell depository.We offer a free testing of reference materials.
  • My cell line is self establihed from a parental cell line / clinical material. Can I confirm its identity?
    • Yes, by co-submitting DNA from the parental cell line or clinical material, we can confirm the identical SNP profile of the matched pairs.
  • My cell line of interest is not included in the database. Can my sample be tested anyway without providing reference material?
    • Yes, the sample can be tested by the human cell line authentication test. The results will indicate whether your cell lines shows a unique SNP-profile or whether your cell line is one of the lines included in the database.
    • You may also try to obtain isolated cell line DNA from a cell depository, e.g. ATCC. We offer a free testing of these reference materials.
  • Can I use cell lysates, used for the McCT Service, as sample for the human cell line authentication?
    • No, it is crucial to use highly purified DNA (see protocol) for the human cell line authentication test.
last update:
03/02/2025
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