Videos and References
A selection of short Youtoube videos about Microscale Thermophoresis
NanoTemper Technology: Learn about the handling of NT.115
short overview about the measurement steps (commercial)
duration: ~ 2 minutes
Measuring Interactions Using Microscale Thermophoresis by NanoTemper
Advantages, applications, physical principle, thermophoresis curves (commercial)
duration ~ 3 minutes
Protein Purification-free Binding Analysis by MicroScale Thermophoresis
purification-free measurement using GFP-tagged proteins
detailed workflow, detailed analysis/curve fitting examples
duration: ~ 10 minutes
Tools
Concentration finder: KD-Intervall Optimization
enter the assumed Kd range for your binding experiment to
find the optimal concentration range of the ligand (titrant).
The tool is available at our Nanotemper machine.
(sorry, no longer available online)
Consumables (capillaries & labelling kits)
Overview about Kits and Capillary Types
product description,
get an a quote for your consumables
The website is unfortunately not as clear as it used to be.
Our machine strictly requires capillaries specified as: Monolith NT.115 ...
Basic Literature
MST Starting Guide – Monolith NT.115 [>]
( i ) concise summary: assay setup and how to avoid common pitfalls
Microscale thermophoresis - Wikipedia [>]
( i ) short introduction into the methodology
MicroScale Thermophoresis: Interaction analysis and beyond [>]
( i ) more detailed introduction into MicroScale Thermophoresis
showing a row of interesting examples
« back to biomolecular interaction