ATAC-Seq

The Assay for Transposase-Accessible Chromatin (ATAC) enables to map open chromatin and generate footprints of nucleosome and transcription factor occupancy in low cell numbers or even single cells. (i) A transposome is generated from a hyperactive Tn5 transposase and a double-stranded DNA adapter. (ii) Tagmentation of a cell lysate using the transposome cuts the chromatin at its accessible positions and appends the adapter to the fragments. (iii) Low cycle number PCR generates a sequencing library. (iv) The library is analysed by next generation sequencing.

Reference:

Buenrostro JD, Giresi PG, Zaba LC, Chang HY, Greenleaf WJ. Transposition of native chromatin for fast and sensitive epigenomic profiling of open chromatin, DNA-binding proteins and nucleosome position. Nat Methods. 2013; 10: 1213-8. Abstract