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Research
Research Topics
Functional and Structural Genomics
Molecular Genetics

Research

Oncogenomics

Fig.1
© dkfz.de

In order to identify the critical genes which are involved in the development of malignant cells we analyze chromosomal aberrations in clinically well defined tumor samples by means of fluorescence in situ hybridization (FISH) (Fig. 1 A, B). Furthermore, we use Comparative Genomic Hybridization (CGH) as a genomic screening approach in order to identify recurrent numerical chromosomal changes, by which oncogenes or tumorsupressor genes could be activated (1c). The results of our analyses are complemented in our department by further approaches, like Matrix-CGH, expression profiling, functional tumor genetics and bioinformatic analysis.

Fig.1: Analysis of chromosomal changes in tumor cell genomes by fluorescence in-situ hybridization (FISH). (A) FISH on a metaphase spread of Hodgkin lymphoma cells. The example shows the duplication of a region of chromosome 2 carrying the c-Rel oncogene. (B) FISH analysis on interphase nuclei (interphase cytogenetics). An experiment on interphase nuclei from chronic lymphocytic leucaemia (CLL) cells is shown. Two different DNA probes, one from a locus on chromosome 7 (red) and one from the locus of tumorsuppressor gene RB-1 (green) were hybridized. While the chromosome 7 derived probe does not show a numerical change (i.e. 2 copies per cell) the RB-1 probe shows only 1 signal, i.e. the RB-1 locus is deleted in these cells. (C) Example of a CGH experiment. Total genomic DNA of tumor cells (green) together with a differently labelled genomic control DNA from normal cells (red) is hybridized on normal metaphase chromosomes. Chromosomal gains or losses are assessed by the hybridization intensity ratio between tumor and normal DNA. Chromosomal gains within the tumor cells result in an increased intensity ratio between tumor and control DNA (greenish staining), losses in a decreased ratio between tumor and control DNA (reddish staining) of the corresponding target chromosomes.

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