Stephanie Hoppe
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Scientific CV
| Jul - Nov 2015 | Postdoctoral scientist, Junior Research Group Immunotherapy and Immunoprevention, DKFZ, Heidelberg |
| 2010 - 2015 | PhD student, Junior Research Group Immunotherapy and -prevention, DKFZ, Heidelberg |
| 2008 - 2010 | MSc Molecular Biosciences, Major Infectious Diseases, Ruprecht-Karls-University, Heidelberg |
| Oct 2009 - Feb 2010 | ERASMUS exchange term, MSc Biomedicine, Karolinska Institutet, Stockholm, Sweden |
| 2005 - 2008 | BSc Biology, Philipps-University, Marburg |
Research Project
Identification of HPV16 T cell epitopes for HLA-A3, HLA-A11 and HLA-A24
The viral oncoproteins E6 and E7 are consistently expressed and required for maintenance of the malignant phenotype in human papillomavirus (HPV)-associated cancers. This makes them ideal targets for immunotherapy. It is known that cell-mediated immune responses are needed for clearing HPV infection. Peptide vaccination specifically elicits cytotoxic T cells (CTL), but is limited by the fact that T cell epitopes are HLA-restricted. A therapeutic vaccine that is applicable to everyone without prior HLA typing needs to contain epitopes for all HLA (super)types.
In this context, the goal of this project was to identify peptides for the HLA-A3/A11 and A24 supertypes from the most common oncogenic HPV type, HPV-16. To this end, HPV-16 E6 and E7 epitopes were predicted. Peptide binding assays for the above mentioned supertypes were performed, as well as specific immunoprecipitations. HPV-16 transformed cell lines of the respective HLA background were analyzed for prospective epitope presence by our targeted mass spectrometry approach. Identified peptides were tested for immunogenicity and their ability to induce CTL.
Resulting Publications
Bonsack M, Hoppe S, Winter J, Tichy D, Zeller C, Küpper MD, Schitter EC, Blatnik R, Riemer AB.
Blatnik R, Mohan N, Bonsack M, Falkenby LG, Hoppe S, Josef K, Steinbach A, Becker S, Nadler WM, Rucevic M, Larsen MR, Salek M, Riemer AB.
Steinbach A, Winter J, Reuschenbach M, Blatnik R, Klevenz A, Bertrand M, Hoppe S, von Knebel Doeberitz M, Grabowska AK, Riemer AB.
Dekhtiarenko I, Ratts RB, Blatnik R, Lee LN, Fischer S, Borkner L, Oduro JD, Marandu TF, Hoppe S, Ruzsics Z, Sonnemann JK, Mansouri M, Meyer C, Lemmermann NA, Holtappels R, Arens R, Klenerman P, Früh K, Reddehase MJ, Riemer AB, Cicin-Sain L.