Cellular Tools
Dr. Rainer Will
The Cellular Tools Core offers standardized vector and clone resources as well as modular services for cell-based gain- and loss-of-function technologies. All service modules are offered as stand-alone services, in combination, or as a complete service pipeline, to give you a maximum of flexibility and support.

Services


Toolbox
Central collection of sequence-verified vectors and clones, including human ORF clones from the international ORFeome Collaboration.
- ~ 70% of all protein-coding human genes (Open Reading Frames - ORFs) in a universal cloning format (Gateway®)
- > 80 different expression vectors expression vectors for mammalian, insect, yeast, and E.coli expression.
- > 50 Halo-Tag & luciferase reporter vectors for protein tagging
- > 40 dual luciferase reporters to quantify activities of oncogenic pathways
- For technical reasons we process orders weekly on Wednesday. Clone & Vector orders placed before are usually ready for pickup Thursday or Friday.

Toolbox
Shuttling of constructs in universal gateway cloning format into highly validated lenti- and retroviral backbones or into expression vectors of choice
- shuttling of your ORFs, miRNAs, shRNAs, etc. of choice into appropriate Destination vectors for mammalian, viral, insect and yeast expression
- provision of with validated bacterial strains, transfection ready DNA and vector maps

Toolbox
Customized generation of stable cell lines by retro- or lentiviral transduction including biosafety and documentation in close interaction with the biological safety department
- generation of retro- and lentiviral particles
- viral transduction of target cell lines
- complete administration of biosafety procedures and full documentation (BIDS)
- S1 in and S1 out – we stably express your required construct in the cell line of your choice
- We are offering a panel of fully sequenced lenti- or retroviral expression vectors
More information about the virus transduction service and the generation of viral particles click here
- standardized cell lines, with save harbor integration (AAVS1 human; Rosa26 mouse) for functional analysis and high throughput screening
- You select your gene of interest, we integrate the ORF into your cell line of choice for stable expression of the encoded protein. We provide you with the cell line with verified (Sanger sequencing) integration sites.
- Information on Stable isogenic recombinant (Sir) cell lines here
Toolbox
Deposition and expansion of single cells under low-pressure conditions and documentation clonality. Optional sorting of cells based on green fluorescence.
- Dispensing of single cells into 96 – or 384 well plates with excellent cell viability and zero crosscontamination
- FDA approved assurance of clonality for each dispensed cell line during spotting runs
- Low throughput sorting of green fluorescent single cells based on fluorescence intensities
- Full reporting of procedures and QC.
Toolbox
Custom generation of knock-out cell lines based on in frame deletion or insertions using synthetic guide RNAs and SpCas9 protein. Knock-in of small tags (~ 20 AA) using single strand oligonucleotides
- sgRNA and SpCas9 protein based Genome Editing Service based on the generation of frameshift insertions / deletions
- Provision of bulk cell lines or of individual clones
- Validation of frameshift mutations by Sanger sequencing and Inference of CRISPR Edits ICE analyses (on request)
- Reporting of procedures and QC.
Team
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Dr. Rainer Will
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Katja Bauer
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Daniela Heiss
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Rita Schatten
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Heike Wilhelm
Publications
Kourtesakis A, Bailey E, Chow HNH, Rohdjeß H, Mussnig N, Agardy DA, Hoffmann DCF, Chih YC, Will R, Kaulen L, Hahn M, Wagener R, Reibold D, Pusch S, Sahm F, Sauer T, Schmitt M, Bunse L, Platten M, Wick W, Kessler T.
Rajagopal V, Seiler J, Nasa I, Cantarella S, Theiss J, Herget F, Kaifer B, Klostermann M, Will R, Schneider M, Helm D, König J, Zarnack K, Diederichs S, Kettenbach AN, Caudron-Herger M.
Jin JX, Fuchslocher F, Carreno-Gonzalez M, Zahnow F, Ceranski AK, Will R, Helm D, Bestvater F, Banito A, Imle R, Ohmura S, Cidre-Aranaz F, Grünewald TGP.
Zhu Y, Kim SN, Chen ZR, Will R, Zhong RD, Dammann P, Sure U.
Hai L, Hoffmann DC, Wagener RJ, Azorin DD, Hausmann D, Xie R, Huppertz MC, Hiblot J, Sievers P, Heuer S, Ito J, Cebulla G, Kourtesakis A, Kaulen LD, Ratliff M, Mandelbaum H, Jung E, Jabali A, Horschitz S, Ernst KJ, Reibold D, Warnken U, Venkataramani V, Will R, Suvà ML, Herold-Mende C, Sahm F, Winkler F, Schlesner M, Wick W, Kessler T.
Beumers L, Vlachavas EI, Borgoni S, Schwarzmüller L, Penso-Dolfin L, Michels BE, Sofyali E, Burmester S, Heiss D, Wilhelm H, Yarden Y, Helm D, Will R, Goncalves A, Wiemann S.
Martija AA, Krauß A, Bächle N, Doth L, Christians A, Krunic D, Schneider M, Helm D, Will R, Hartmann C, Herold-Mende C, von Deimling A, Pusch S.
Krämer C, Kilian M, Chih YC, Kourtesakis A, Hoffmann DC, Boschert T, Koopmann P, Sanghvi K, De Roia A, Jung S, Jähne K, Day B, Shultz LD, Ratliff M, Harbottle R, Green EW, Will R, Wick W, Platten M, Bunse L.
Chen Z, Will R, Kim SN, Busch MA, Dünker N, Dammann P, Sure U, Zhu Y.
Pane AA, Kordaß T, Hotz-Wagenblatt A, Dickes E, Kopp-Schneider A, Will R, Seliger B, Osen W, Eichmüller SB.
Kim M, Reidenbach S, Schlechter T, Rothmann AC, Will R, Hofmann I.
Hübers C, Abdul Pari AA, Grieshober D, Petkov M, Schmidt A, Messmer T, Heyer CM, Schölch S, Kapel SS, Gengenbacher N, Singhal M, Schieb B, Fricke C, Will R, Remans K, Utikal JS, Reissfelder C, Schlesner M, Hodivala-Dilke KM, Kersten S, Goerdt S, Augustin HG, Felcht M.
Noronha A, Belugali Nataraj N, Lee JS, Zhitomirsky B, Oren Y, Oster S, Lindzen M, Mukherjee S, Will R, Ghosh S, Simoni-Nieves A, Verma A, Chatterjee R, Borgoni S, Robinson W, Sinha S, Brandis A, Kerr DL, Wu W, Sekar A, Giri S, Chung Y, Drago-Garcia D, Danysh BP, Lauriola M, Fiorentino M, Ardizzoni A, Oren M, Blakely CM, Ezike J, Wiemann S, Parida L, Bivona TG, Aqeilan RI, Brugge JS, Regev A, Getz G, Ruppin E, Yarden Y.
Li X, Michels BE, Tosun OE, Jung J, Kappes J, Ibing S, Nataraj NB, Sahay S, Schneider M, Wörner A, Becki C, Ishaque N, Feuerbach L, Heßling B, Helm D, Will R, Yarden Y, Müller-Decker K, Wiemann S, Körner C.
Will R, Bauer K, Kudla M, Montero-Vergara J, Wiemann S, Jendrossek V, Peña-Llopis S, Vega-Rubín-de-Celis S.
Giacomelli C, Jung J, Wachter A, Ibing S, Will R, Uhlmann S, Mannsperger H, Sahin Ö, Yarden Y, Beißbarth T, Korf U, Körner C, Wiemann S.
FYCO1 Regulates Cardiomyocyte Autophagy and Prevents Heart Failure Due to Pressure Overload In Vivo.
Kuhn C, Menke M, Senger F, Mack C, Dierck F, Hille S, Schmidt I, Brunke G, Bünger P, Schmiedel N, Will R, Sossalla S, Frank D, Eschenhagen T, Carrier L, Lüllmann-Rauch R, Rangrez AY, Frey N.
Maia A, Gu Z, Koch A, Berdiel-Acer M, Will R, Schlesner M, Wiemann S.
Hartmann L, Schröter P, Osen W, Baumann D, Offringa R, Moustafa M, Will R, Debus J, Brons S, Rieken S, Eichmüller SB.
Borgoni S, Sofyalı E, Soleimani M, Wilhelm H, Müller-Decker K, Will R, Noronha A, Beumers L, Verschure PJ, Yarden Y, Magnani L, van Kampen AHC, Moerland PD, Wiemann S.
Martin-Garcia D, Silva-Vilches C, Will R, Enk AH, Lonsdorf AS.
Haller F, Bieg M, Will R, Körner C, Weichenhan D, Bott A, Ishaque N, Lutsik P, Moskalev EA, Mueller SK, Bähr M, Woerner A, Kaiser B, Scherl C, Haderlein M, Kleinheinz K, Fietkau R, Iro H, Eils R, Hartmann A, Plass C, Wiemann S, Agaimy A.
Quandt J, Schlude C, Bartoschek M, Will R, Cid-Arregui A, Schölch S, Reissfelder C, Weitz J, Schneider M, Wiemann S, Momburg F, Beckhove P.
Breunig C, Pahl J, Küblbeck M, Miller M, Antonelli D, Erdem N, Wirth C, Will R, Bott A, Cerwenka A, Wiemann S.
Brocks D, Schmidt CR, Daskalakis M, Jang HS, Shah NM, Li D, Li J, Zhang B, Hou Y, Laudato S, Lipka DB, Schott J, Bierhoff H, Assenov Y, Helf M, Ressnerova A, Islam MS, Lindroth AM, Haas S, Essers M, Imbusch CD, Brors B, Oehme I, Witt O, Lübbert M, Mallm JP, Rippe K, Will R, Weichenhan D, Stoecklin G, Gerhäuser C, Oakes CC, Wang T, Plass C.
Goeppert B, Ernst C, Baer C, Roessler S, Renner M, Mehrabi A, Hafezi M, Pathil A, Warth A, Stenzinger A, Weichert W, Bähr M, Will R, Schirmacher P, Plass C, Weichenhan D.
Haller F, Knopf J, Ackermann A, Bieg M, Kleinheinz K, Schlesner M, Moskalev EA, Will R, Satir AA, Abdelmagid IE, Giedl J, Carbon R, Rompel O, Hartmann A, Wiemann S, Metzler M, Agaimy A.
Shukla K, Sharma AK, Ward A, Will R, Hielscher T, Balwierz A, Breunig C, Münstermann E, König R, Keklikoglou I, Wiemann S.
Cohen-Dvashi H, Ben-Chetrit N, Russell R, Carvalho S, Lauriola M, Nisani S, Mancini M, Nataraj N, Kedmi M, Roth L, Köstler W, Zeisel A, Yitzhaky A, Zylberg J, Tarcic G, Eilam R, Wigelman Y, Will R, Lavi S, Porat Z, Wiemann S, Ricardo S, Schmitt F, Caldas C, Yarden Y.