Cancer Epigenomics

Deregulation of mechanisms that control the establishment of normal DNA methylation patterns leads to both extensive aberrant hypo- and hypermethylation and has been described for several human malignancies. Global DNA hypomethylation in human cancers was one of the earliest changes associated with tumor progression. Our group has shown that human malignancies are characterized by extensive promoter CpG island methylation with non-random and tumor-type specific patterns. DNA methylation changes co-occur with other epigenetic alterations such as nucleosome positioning and histone tail modifications. It is to the greatest extent unknown how tumors acquire aberrant DNA methylation patterns, however many of the enzymes that regulate epigenetic processes are mutated in human malignancies. Our Division is interested in the molecular mechanisms underlying the initiation and progression of malignant cell growth. In particular, we are focusing our attention on the contribution of epigenetic alterations in this process and to determine how epigenetic and genetic alterations cooperate during tumorigenesis. In our studies we are utilizing current state-of-the-art high throughput epigenomic assays (e.g. Illumina methylation arrays, next-generation whole genome bisulfite sequencing on minute amounts of cells, sc-multiome sequencing, antibody-guided chromatin tagmentation (ACT-seq)) on clinical samples, cell culture and rodent tumor models.

Future Outlook

Epigenetics is a quickly evolving field with links to many research directions in cancer research. A challenge here will be to integrate epigenetic questions with other data sets. For example in the past, the profiling of cancer genomes relied heavily on the description of genetic alterations. Now, epigenetic datasets will need to be included in order to completely understand the molecular defects in cancer. Furthermore, cellular identity is achieved through epigenetic regulation, and we will need to better define the epigenomes of cell types from which cancers arise since these preexisting epigenomes will influence the progression and aggressiveness of cancers as well as influence the therapeutic outcome. Our Division will focus on six major research directions:

• Evaluation of genome-wide epigenetic patterns in tumor genomes and the cell of origin from which individual cancers arise
• Identification of novel cancer genes and pathways targeted by epigenetic alterations
• Define the molecular mechanisms that lead to altered epigenetic patterns in cancer
• Determining the role of epigenetic pattern dynamics in differentiation of hematopoietic stem cells and other cell types
• Evaluating the role of epigenetics in the regulation of damage response
• Developing bioinformatical tools for the integrative analysis of epigenetic data